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double antibody sandwich elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology double antibody sandwich elisa kit
    Double Antibody Sandwich Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tumor+necrosis+factor+%CE%B1/High+Sensitivity+Mouse+TNF-%CE%B1+(Tumor+Necrosis+Factor+Alpha)+ELISA+Kit/pm42014724-229-12-17
    Average 95 stars, based on 30 article reviews
    double antibody sandwich elisa kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats.
    Article Snippet: .. To perform the protein blocking to prevent nonspecific binding of antibodies sections were incubated with Ultra V Block solution (cat. no. TA‐125‐UB; Thermo Fisher Scientific, Inc.) for 30 min at room temperature, kidney and lung tissue sections were incubated with anti‐tumor necrosis factor‐α (TNF‐α; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐33121), anti‐interleukin 1β (IL‐1β; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐66749) and anti‐intercellular adhesion molecule 1 (ICAM‐1; 1:100; BIOSS; cat. no. bs‐0608R) primary antibodies to investigate the inflam‐ matory processes. .. Additionally, kidney sections were incubated with anti‐B‐cell lymphoma 2 (BCL‐2)‐associated X protein (BAX; 1:100; BIOSS; cat. no. bs‐0127R), anti‐BCL‐2 (1:200; BIOSS; cat. no. bs‐4563R) and anti‐caspase‐3 (CASP‐3; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐66940) primary antibodies to examine the apoptotic processes.

    Article Title: Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats
    Article Snippet: .. To perform the protein blocking to prevent nonspecific binding of antibodies sections were incubated with Ultra V Block solution (cat. no. TA-125-UB; Thermo Fisher Scientific, Inc.) for 30 min at room temperature, kidney and lung tissue sections were incubated with anti-tumor necrosis factor-α (TNF-α; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-33121), anti-interleukin 1β (IL-1β; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-66749) and anti-intercellular adhesion molecule 1 (ICAM-1; 1:100; BIOSS; cat. no. bs-0608R) primary antibodies to investigate the inflammatory processes. .. Additionally, kidney sections were incubated with anti-B-cell lymphoma 2 (BCL-2)-associated X protein (BAX; 1:100; BIOSS; cat. no. bs-0127R), anti-BCL-2 (1:200; BIOSS; cat. no. bs-4563R) and anti-caspase-3 (CASP-3; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-66940) primary antibodies to examine the apoptotic processes.

    Binding Assay:

    Article Title: Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats.
    Article Snippet: .. To perform the protein blocking to prevent nonspecific binding of antibodies sections were incubated with Ultra V Block solution (cat. no. TA‐125‐UB; Thermo Fisher Scientific, Inc.) for 30 min at room temperature, kidney and lung tissue sections were incubated with anti‐tumor necrosis factor‐α (TNF‐α; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐33121), anti‐interleukin 1β (IL‐1β; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐66749) and anti‐intercellular adhesion molecule 1 (ICAM‐1; 1:100; BIOSS; cat. no. bs‐0608R) primary antibodies to investigate the inflam‐ matory processes. .. Additionally, kidney sections were incubated with anti‐B‐cell lymphoma 2 (BCL‐2)‐associated X protein (BAX; 1:100; BIOSS; cat. no. bs‐0127R), anti‐BCL‐2 (1:200; BIOSS; cat. no. bs‐4563R) and anti‐caspase‐3 (CASP‐3; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐66940) primary antibodies to examine the apoptotic processes.

    Article Title: Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats
    Article Snippet: .. To perform the protein blocking to prevent nonspecific binding of antibodies sections were incubated with Ultra V Block solution (cat. no. TA-125-UB; Thermo Fisher Scientific, Inc.) for 30 min at room temperature, kidney and lung tissue sections were incubated with anti-tumor necrosis factor-α (TNF-α; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-33121), anti-interleukin 1β (IL-1β; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-66749) and anti-intercellular adhesion molecule 1 (ICAM-1; 1:100; BIOSS; cat. no. bs-0608R) primary antibodies to investigate the inflammatory processes. .. Additionally, kidney sections were incubated with anti-B-cell lymphoma 2 (BCL-2)-associated X protein (BAX; 1:100; BIOSS; cat. no. bs-0127R), anti-BCL-2 (1:200; BIOSS; cat. no. bs-4563R) and anti-caspase-3 (CASP-3; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-66940) primary antibodies to examine the apoptotic processes.

    Incubation:

    Article Title: Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats.
    Article Snippet: .. To perform the protein blocking to prevent nonspecific binding of antibodies sections were incubated with Ultra V Block solution (cat. no. TA‐125‐UB; Thermo Fisher Scientific, Inc.) for 30 min at room temperature, kidney and lung tissue sections were incubated with anti‐tumor necrosis factor‐α (TNF‐α; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐33121), anti‐interleukin 1β (IL‐1β; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐66749) and anti‐intercellular adhesion molecule 1 (ICAM‐1; 1:100; BIOSS; cat. no. bs‐0608R) primary antibodies to investigate the inflam‐ matory processes. .. Additionally, kidney sections were incubated with anti‐B‐cell lymphoma 2 (BCL‐2)‐associated X protein (BAX; 1:100; BIOSS; cat. no. bs‐0127R), anti‐BCL‐2 (1:200; BIOSS; cat. no. bs‐4563R) and anti‐caspase‐3 (CASP‐3; 1:100; Elabscience Biotechnology, Inc.; cat. no. E‐AB‐66940) primary antibodies to examine the apoptotic processes.

    Article Title: Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats
    Article Snippet: .. To perform the protein blocking to prevent nonspecific binding of antibodies sections were incubated with Ultra V Block solution (cat. no. TA-125-UB; Thermo Fisher Scientific, Inc.) for 30 min at room temperature, kidney and lung tissue sections were incubated with anti-tumor necrosis factor-α (TNF-α; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-33121), anti-interleukin 1β (IL-1β; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-66749) and anti-intercellular adhesion molecule 1 (ICAM-1; 1:100; BIOSS; cat. no. bs-0608R) primary antibodies to investigate the inflammatory processes. .. Additionally, kidney sections were incubated with anti-B-cell lymphoma 2 (BCL-2)-associated X protein (BAX; 1:100; BIOSS; cat. no. bs-0127R), anti-BCL-2 (1:200; BIOSS; cat. no. bs-4563R) and anti-caspase-3 (CASP-3; 1:100; Elabscience Biotechnology, Inc.; cat. no. E-AB-66940) primary antibodies to examine the apoptotic processes.



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    Image Search Results


    TCE alleviates LPS-induced acute lung injury (ALI) in mice. Effects of TCE on the levels of IL-6 ( A ), TNF-α ( B ), CCL2 ( C ), and IL-1β ( D ) in BALF ( n = 8). ( E ) Lung injury scores were calculated according to the predetermined criteria. ## p < 0.01 vs. negative control group; * p < 0.05 and ** p < 0.01 vs. model control group. ND, not detected. ( F ) Representative images of H&E staining in lung tissues (200×). Edema is indicated by green arrows, while blue arrows denote alveolar and interstitial inflammation. Red arrows highlight alveolar and interstitial hemorrhage, and yellow arrows mark neutrophil infiltration. The yellow pentagrams point to atelectasis. Scale bars = 50 μm. Dexamethasone served as a positive control.

    Journal: Plants

    Article Title: Anti-Complement, Anti-Oxidative, and Anti-Inflammatory Activities of the Ethanol Extract of Tamarix chinensis Lour.

    doi: 10.3390/plants15142199

    Figure Lengend Snippet: TCE alleviates LPS-induced acute lung injury (ALI) in mice. Effects of TCE on the levels of IL-6 ( A ), TNF-α ( B ), CCL2 ( C ), and IL-1β ( D ) in BALF ( n = 8). ( E ) Lung injury scores were calculated according to the predetermined criteria. ## p < 0.01 vs. negative control group; * p < 0.05 and ** p < 0.01 vs. model control group. ND, not detected. ( F ) Representative images of H&E staining in lung tissues (200×). Edema is indicated by green arrows, while blue arrows denote alveolar and interstitial inflammation. Red arrows highlight alveolar and interstitial hemorrhage, and yellow arrows mark neutrophil infiltration. The yellow pentagrams point to atelectasis. Scale bars = 50 μm. Dexamethasone served as a positive control.

    Article Snippet: Antibodies against ERK (Cat # A10613, Lot # 9410613001), GAPDH (Cat # AC033, Lot # 9100033001), and horseradish peroxidase (HRP)-conjugated anti-mouse (Cat # AS003, Lot # 9300033001); anti-rabbit IgG (Cat # AS014, Lot # 9300014001); ColorMixed Protein Marker 180 (10–180 kDa) (Cat # RM19001, Lot # 9623141422); and mouse TNF-α ELISA kit (Cat # RK00027, Lot # 9680058060126) were bought from ABclonal Biotech Co. (Wuhan, Hubei, China).

    Techniques: Negative Control, Control, Staining, Positive Control

    Physical exercise alleviates neuroinflammation and enhances phagocytic activity of microglia after ischemic stroke. (A) Immunoblotting analyses of inflammation-related proteins 21 days after intervention ( n = 5). (B) More Iba1 (red; Alexa Fluor® 568) co-localized with IL-10 (green; Alexa Fluor® 488) in the tMCAO + PE group ( n = 4). Scale bar: 20 μm. (C) More Iba1 (red; Alexa Fluor® 568) co-localized with myelin debris (dMBP) (green; Alexa Fluor® 488) in the tMCAO + PE group ( n = 4). Scale bar: 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s honestly significant difference test). DAPI: 4′,6-Diamidino-2-phenylindole; dMBP: degraded myelin basic protein; Iba1: ionized calcium-binding adapter molecule 1; IL-10: interleukin 10; iNOS: inducible isoform of nitric oxide synthase; PE: physical exercise; Sham: sham-operated; TGF-β: transforming growth factor-beta; tMCAO: transient middle cerebral artery occlusion; TNF-α: tumor necrosis factor-alpha.

    Journal: Neural Regeneration Research

    Article Title: Physical exercise promotes white matter repair after ischemic stroke

    doi: 10.4103/NRR.NRR-D-24-00861

    Figure Lengend Snippet: Physical exercise alleviates neuroinflammation and enhances phagocytic activity of microglia after ischemic stroke. (A) Immunoblotting analyses of inflammation-related proteins 21 days after intervention ( n = 5). (B) More Iba1 (red; Alexa Fluor® 568) co-localized with IL-10 (green; Alexa Fluor® 488) in the tMCAO + PE group ( n = 4). Scale bar: 20 μm. (C) More Iba1 (red; Alexa Fluor® 568) co-localized with myelin debris (dMBP) (green; Alexa Fluor® 488) in the tMCAO + PE group ( n = 4). Scale bar: 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s honestly significant difference test). DAPI: 4′,6-Diamidino-2-phenylindole; dMBP: degraded myelin basic protein; Iba1: ionized calcium-binding adapter molecule 1; IL-10: interleukin 10; iNOS: inducible isoform of nitric oxide synthase; PE: physical exercise; Sham: sham-operated; TGF-β: transforming growth factor-beta; tMCAO: transient middle cerebral artery occlusion; TNF-α: tumor necrosis factor-alpha.

    Article Snippet: The primary antibodies used in this study were: anti-inducible isoform of nitric oxide synthase (iNOS; rabbit mAb, 1:1000, Abclonal, Cat# A3774, RRID: AB_3094627), anti-tumor necrosis factor-alpha (TNF-α; rabbit pAb, 1:1000, Abclonal, Cat# A23264, RRID: AB_3665498), anti-transforming growth factor-beta (TGF-β; mouse mAb, 1:1000, Affinity, Liyang, China, Cat# BF8012, RRID: AB_3665500), anti-IL-10 (rabbit mAb, 1:1000, Abclonal, Cat# A12255, RRID: AB_3665480), anti-β-tubulin (mouse mAb, 1:1000, Affinity, Cat# T0023, RRID: AB_2813772), anti-OPN (mouse mAb, 1:1000, Abmart, Shanghai, China, Cat# MS20111, RRID: AB_3665504), and anti-C-X-C motif chemokine ligand 12 (CXCL12; rabbit Ab, 1:1000, Abmart, Cat# PK12227, RRID: AB_3665506).

    Techniques: Activity Assay, Western Blot, Binding Assay

    Treg cells are essential for physical exercise-induced reduction of inflammation and promotion of phagocytosis after ischemic stroke. (A) Immunoblotting analyses of inflammation-related proteins 21 days after intervention ( n = 4). (B) More Iba1 (red; Alexa Fluor® 568) co-localized with IL-10 (green; Alexa Fluor® 488) in the PE + IgG group ( n = 4). Scale bar: 20 μm. (C) More Iba1 (red; Alexa Fluor® 568) co-localized with myelin debris (dMBP) (green; Alexa Fluor® 488) in the PE + IgG group ( n = 4). Scale bar: 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (unpaired t -test). DAPI: 4′,6-Diamidino-2-phenylindole; dMBP: degraded myelin basic protein; Iba1: ionized calcium-binding adapter molecule 1; IL-10: interleukin 10; iNOS: inducible isoform of nitric oxide synthase; PE: physical exercise; TGF-β: transforming growth factor-beta; TNF-α: tumor necrosis factor-alpha; Treg cell: regulatory T cell.

    Journal: Neural Regeneration Research

    Article Title: Physical exercise promotes white matter repair after ischemic stroke

    doi: 10.4103/NRR.NRR-D-24-00861

    Figure Lengend Snippet: Treg cells are essential for physical exercise-induced reduction of inflammation and promotion of phagocytosis after ischemic stroke. (A) Immunoblotting analyses of inflammation-related proteins 21 days after intervention ( n = 4). (B) More Iba1 (red; Alexa Fluor® 568) co-localized with IL-10 (green; Alexa Fluor® 488) in the PE + IgG group ( n = 4). Scale bar: 20 μm. (C) More Iba1 (red; Alexa Fluor® 568) co-localized with myelin debris (dMBP) (green; Alexa Fluor® 488) in the PE + IgG group ( n = 4). Scale bar: 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (unpaired t -test). DAPI: 4′,6-Diamidino-2-phenylindole; dMBP: degraded myelin basic protein; Iba1: ionized calcium-binding adapter molecule 1; IL-10: interleukin 10; iNOS: inducible isoform of nitric oxide synthase; PE: physical exercise; TGF-β: transforming growth factor-beta; TNF-α: tumor necrosis factor-alpha; Treg cell: regulatory T cell.

    Article Snippet: The primary antibodies used in this study were: anti-inducible isoform of nitric oxide synthase (iNOS; rabbit mAb, 1:1000, Abclonal, Cat# A3774, RRID: AB_3094627), anti-tumor necrosis factor-alpha (TNF-α; rabbit pAb, 1:1000, Abclonal, Cat# A23264, RRID: AB_3665498), anti-transforming growth factor-beta (TGF-β; mouse mAb, 1:1000, Affinity, Liyang, China, Cat# BF8012, RRID: AB_3665500), anti-IL-10 (rabbit mAb, 1:1000, Abclonal, Cat# A12255, RRID: AB_3665480), anti-β-tubulin (mouse mAb, 1:1000, Affinity, Cat# T0023, RRID: AB_2813772), anti-OPN (mouse mAb, 1:1000, Abmart, Shanghai, China, Cat# MS20111, RRID: AB_3665504), and anti-C-X-C motif chemokine ligand 12 (CXCL12; rabbit Ab, 1:1000, Abmart, Cat# PK12227, RRID: AB_3665506).

    Techniques: Western Blot, Binding Assay

    Osteopontin may be the molecular bridge for Treg regulation of microglial function after ischemic stroke. (A) Immunoblotting analyses of OPN 21 days after intervention ( n = 4–5). (B) Experimental design for mRNA extraction from Treg cells. (C) mRNA expression of Spp1 in Treg cells. (D) Experimental design for BV2 microglia in vitro . Created with Microsoft PowerPoint 2019. (E) Expression of inflammation-related genes. (F) More fluorescent microbeads (green) co-localized with microglia (Iba1 + ) (red; Alexa Fluor® 555). Scale bar: 50 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s honestly significant difference test). CM: Conditioned medium; DAPI: 4′,6-diamidino-2-phenylindole; Iba1: ionized calcium-binding adapter molecule 1; IL-10: interleukin 10; iNOS: inducible isoform of nitric oxide synthase; OGD/R: oxygen glucose deprivation/re-oxygenation; OPN: osteopontin; PE: physical exercise; qPCR: quantitative polymerase chain reaction; TGF-β: transforming growth factor-beta; tMCAO: transient middle cerebral artery occlusion; TNF-α: tumor necrosis factor-alpha; Treg cell: regulatory T cell.

    Journal: Neural Regeneration Research

    Article Title: Physical exercise promotes white matter repair after ischemic stroke

    doi: 10.4103/NRR.NRR-D-24-00861

    Figure Lengend Snippet: Osteopontin may be the molecular bridge for Treg regulation of microglial function after ischemic stroke. (A) Immunoblotting analyses of OPN 21 days after intervention ( n = 4–5). (B) Experimental design for mRNA extraction from Treg cells. (C) mRNA expression of Spp1 in Treg cells. (D) Experimental design for BV2 microglia in vitro . Created with Microsoft PowerPoint 2019. (E) Expression of inflammation-related genes. (F) More fluorescent microbeads (green) co-localized with microglia (Iba1 + ) (red; Alexa Fluor® 555). Scale bar: 50 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s honestly significant difference test). CM: Conditioned medium; DAPI: 4′,6-diamidino-2-phenylindole; Iba1: ionized calcium-binding adapter molecule 1; IL-10: interleukin 10; iNOS: inducible isoform of nitric oxide synthase; OGD/R: oxygen glucose deprivation/re-oxygenation; OPN: osteopontin; PE: physical exercise; qPCR: quantitative polymerase chain reaction; TGF-β: transforming growth factor-beta; tMCAO: transient middle cerebral artery occlusion; TNF-α: tumor necrosis factor-alpha; Treg cell: regulatory T cell.

    Article Snippet: The primary antibodies used in this study were: anti-inducible isoform of nitric oxide synthase (iNOS; rabbit mAb, 1:1000, Abclonal, Cat# A3774, RRID: AB_3094627), anti-tumor necrosis factor-alpha (TNF-α; rabbit pAb, 1:1000, Abclonal, Cat# A23264, RRID: AB_3665498), anti-transforming growth factor-beta (TGF-β; mouse mAb, 1:1000, Affinity, Liyang, China, Cat# BF8012, RRID: AB_3665500), anti-IL-10 (rabbit mAb, 1:1000, Abclonal, Cat# A12255, RRID: AB_3665480), anti-β-tubulin (mouse mAb, 1:1000, Affinity, Cat# T0023, RRID: AB_2813772), anti-OPN (mouse mAb, 1:1000, Abmart, Shanghai, China, Cat# MS20111, RRID: AB_3665504), and anti-C-X-C motif chemokine ligand 12 (CXCL12; rabbit Ab, 1:1000, Abmart, Cat# PK12227, RRID: AB_3665506).

    Techniques: Western Blot, Extraction, Expressing, In Vitro, Binding Assay, Real-time Polymerase Chain Reaction

    Probiotics improved atrial fibrillation (AF) by regulating intestinal flora imbalance and the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome signaling pathway in rats. The expression of AF biomarkers pentraxin 3 (PTX3), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), chemerin, and Gal-3 was detected by Western blot (A) and real-time quantitative polymerase chain reaction (RT-qPCR) (B). n = 5; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Probiotics Ameliorate Atrial Fibrillation-Associated Biomarkers and Inflammation in Rats via Modulation of the Gut Microbiota and NLRP3 Inflammasome Pathway

    doi: 10.5812/ijpr-168612

    Figure Lengend Snippet: Probiotics improved atrial fibrillation (AF) by regulating intestinal flora imbalance and the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome signaling pathway in rats. The expression of AF biomarkers pentraxin 3 (PTX3), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), chemerin, and Gal-3 was detected by Western blot (A) and real-time quantitative polymerase chain reaction (RT-qPCR) (B). n = 5; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The membranes were blocked with 5% skim milk at room temperature for 1 h and subsequently incubated overnight at 4°C with the following primary antibodies: Anti-NLRP3 antibody (68102-1-lg, Proteintech), Anti-caspase-1 antibody (HA722222, Huane Bio), Anti-IL-1β antibody (26048-1-ap, Proteintech), Anti-pentraxin 3 (PTX3) antibody (DF8762, Affinity), Anti-IL-6 antibody (ab9324, abcam), Anti-tumor necrosis factor-alpha (TNF-α) antibody (bs-0549R, Bioss), Anti-chemerin antibody (833550-4-RR, Proteintech), Anti-Galectin-3 antibody (60207-1-Ig, Proteintech), and Anti-GAPDH antibody (AF7021, Affinity).

    Techniques: Probiotics, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    FXR and HDCA regulates the levels of intestinal inflammation. (A) The relative expression of mRNA level in the distal ileum (n = 6). (B) Representative immunoblots of proteins in the distal ileum (n = 3). Relative expression levels of NLRP3 (C), IL-6 (D), TNF-α (E), IL-18 (F), IL-10 (G), IL-1β p17 (H). (I) Immunohistochemical staining and (J) quantitative analysis of NLRP3+, IL-6+, TNF-α+ and IL-18+ cells in the distal ileum (scale bar, 100 μm, n = 6). * P < 0.05, ** P < 0.01 by One-way ANOVA with post hoc Tukey's test (A, C–H, J). Data is presented as mean ± SEM.

    Journal: Journal of Advanced Research

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling

    doi: 10.1016/j.jare.2025.07.017

    Figure Lengend Snippet: FXR and HDCA regulates the levels of intestinal inflammation. (A) The relative expression of mRNA level in the distal ileum (n = 6). (B) Representative immunoblots of proteins in the distal ileum (n = 3). Relative expression levels of NLRP3 (C), IL-6 (D), TNF-α (E), IL-18 (F), IL-10 (G), IL-1β p17 (H). (I) Immunohistochemical staining and (J) quantitative analysis of NLRP3+, IL-6+, TNF-α+ and IL-18+ cells in the distal ileum (scale bar, 100 μm, n = 6). * P < 0.05, ** P < 0.01 by One-way ANOVA with post hoc Tukey's test (A, C–H, J). Data is presented as mean ± SEM.

    Article Snippet: Membranes were blocked using 5 % skim milk at room temperature for 1 h and subsequently incubated at 4 °C overnight with primary antibodies against FXR (1:1000, Immunoway, YN2161), zonula occludens-1 (ZO-1) (1:1000, Affinity, AF5145), Mucin-2 (1:1000, Abcam, ab272692), occludin (1:1000, Proteintech, 27260–1-AP), claudin-1 (1:1000, WL, WL03073), MMP-2 (1:1000, Immunoway, YT2798), MMP-9 (1:1000, Immunoway, YT1892), PPAR-γ (1:1000, Proteintech, 16643-1-AP), NOD-like receptor protein-3 (NLRP3) (1:1000, Immunoway, YT5382), interleukin-6 (IL-6) (1:1000, WL, WL02841), IL-18 (1:1000, WL, WL01127), tumor necrosis factor-alpha (TNF-α) (1:1000, WL, WL01581), IL-10 (1:1000, WL, WL03088), FGF15 (1:500, Santacruz, sc-514647), TGR5 (1:1000, Abcam, ab72608), BSEP (1:1000, Abcam, ab155421), CYP7α1 (1:1000, Abcam, ab65596) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, 60004-1-Ig).

    Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining

    HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of CD86 in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.

    Journal: Journal of Advanced Research

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling

    doi: 10.1016/j.jare.2025.07.017

    Figure Lengend Snippet: HDCA regulates the inflammatory processes in the spinal cord. (A) Representative immunofluorescence staining results of IBA-1 in the spinal cord (scale, 100 μm, n = 3). (B) Immunofluorescence staining of CD86 in the spinal cord (scale, 100 μm, n = 3). (C) The mean fluorescence intensity of IBA-1 in spinal cord (n = 3). (D) The mean fluorescence intensity of CD86 in spinal cord (n = 3). (E) Relative expression of mRNA level in spinal cord (n = 6). (F) Representative immunoblots protein in spinal cord. (G) Relative expression levels of NLRP3, IL-10, IL-18, TNF-α protein (n = 3). * P < 0.05, ** P < 0.01by One-way ANOVA with post hoc Tukey's test (C, D, E, G). Data is presented as mean ± SEM.

    Article Snippet: Membranes were blocked using 5 % skim milk at room temperature for 1 h and subsequently incubated at 4 °C overnight with primary antibodies against FXR (1:1000, Immunoway, YN2161), zonula occludens-1 (ZO-1) (1:1000, Affinity, AF5145), Mucin-2 (1:1000, Abcam, ab272692), occludin (1:1000, Proteintech, 27260–1-AP), claudin-1 (1:1000, WL, WL03073), MMP-2 (1:1000, Immunoway, YT2798), MMP-9 (1:1000, Immunoway, YT1892), PPAR-γ (1:1000, Proteintech, 16643-1-AP), NOD-like receptor protein-3 (NLRP3) (1:1000, Immunoway, YT5382), interleukin-6 (IL-6) (1:1000, WL, WL02841), IL-18 (1:1000, WL, WL01127), tumor necrosis factor-alpha (TNF-α) (1:1000, WL, WL01581), IL-10 (1:1000, WL, WL03088), FGF15 (1:500, Santacruz, sc-514647), TGR5 (1:1000, Abcam, ab72608), BSEP (1:1000, Abcam, ab155421), CYP7α1 (1:1000, Abcam, ab65596) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, 60004-1-Ig).

    Techniques: Immunofluorescence, Staining, Fluorescence, Expressing, Western Blot

    Histology and IL-2 expression in rat hemorrhoidal tissue. A Microscopic appearance of the hemorrhoid areas of the rats in each group (HE, ×50; a model group, b control group). B Expression of IL-2 in anorectal tissue in each group detected by immunohistochemical staining. (IHC, ×50; a model group, b control group)

    Journal: Techniques in Coloproctology

    Article Title: Comparison of the modeling effects between two hemorrhoid models

    doi: 10.1007/s10151-026-03303-x

    Figure Lengend Snippet: Histology and IL-2 expression in rat hemorrhoidal tissue. A Microscopic appearance of the hemorrhoid areas of the rats in each group (HE, ×50; a model group, b control group). B Expression of IL-2 in anorectal tissue in each group detected by immunohistochemical staining. (IHC, ×50; a model group, b control group)

    Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

    Techniques: Expressing, Control, Immunohistochemical staining, Staining

    Immunohistochemical overview of anorectal cytokine expression. Expression of A IL-2, B IL-6, C , and D TNFα in anorectal tissue in each group detected by immunohistochemical staining (IHC, ×50; a model group, b control group)

    Journal: Techniques in Coloproctology

    Article Title: Comparison of the modeling effects between two hemorrhoid models

    doi: 10.1007/s10151-026-03303-x

    Figure Lengend Snippet: Immunohistochemical overview of anorectal cytokine expression. Expression of A IL-2, B IL-6, C , and D TNFα in anorectal tissue in each group detected by immunohistochemical staining (IHC, ×50; a model group, b control group)

    Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

    Techniques: Immunohistochemical staining, Expressing, Staining, Control

    Expression of TNFα in the anorectal tissue in each group detected by immunohistochemical staining. * P < 0.05 vs control group

    Journal: Techniques in Coloproctology

    Article Title: Comparison of the modeling effects between two hemorrhoid models

    doi: 10.1007/s10151-026-03303-x

    Figure Lengend Snippet: Expression of TNFα in the anorectal tissue in each group detected by immunohistochemical staining. * P < 0.05 vs control group

    Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

    Techniques: Expressing, Immunohistochemical staining, Staining, Control

    Relative mRNA expression of pro-inflammatory cytokines A IL-2, B IL-6, and C TNFα in anorectal tissue in each group. * P < 0.05 vs control group

    Journal: Techniques in Coloproctology

    Article Title: Comparison of the modeling effects between two hemorrhoid models

    doi: 10.1007/s10151-026-03303-x

    Figure Lengend Snippet: Relative mRNA expression of pro-inflammatory cytokines A IL-2, B IL-6, and C TNFα in anorectal tissue in each group. * P < 0.05 vs control group

    Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

    Techniques: Expressing, Control

    Western blotting for detecting protein expression of IL-2, IL-6, and TNFα

    Journal: Techniques in Coloproctology

    Article Title: Comparison of the modeling effects between two hemorrhoid models

    doi: 10.1007/s10151-026-03303-x

    Figure Lengend Snippet: Western blotting for detecting protein expression of IL-2, IL-6, and TNFα

    Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

    Techniques: Western Blot, Expressing

    IL-2, IL-6, and TNFα protein levels in anorectal tissue are significantly elevated in the model group compared to the control group (* P < 0.05). A IL-2, B IL-6, and C TNFα protein expression in anorectal tissue. * P < 0.05 vs control group

    Journal: Techniques in Coloproctology

    Article Title: Comparison of the modeling effects between two hemorrhoid models

    doi: 10.1007/s10151-026-03303-x

    Figure Lengend Snippet: IL-2, IL-6, and TNFα protein levels in anorectal tissue are significantly elevated in the model group compared to the control group (* P < 0.05). A IL-2, B IL-6, and C TNFα protein expression in anorectal tissue. * P < 0.05 vs control group

    Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

    Techniques: Control, Expressing